human cd25 il2 r alpha duoset elisa kit (R&D Systems)

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R&D Systems human cd25 il2 r alpha duoset elisa kit
Human Cd25 Il2 R Alpha Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cd25+il2+r+alpha+elisa+kit/Human+CD25%2FIL-2R+alpha+DuoSet+ELISA/pmc06945765-235-11-18
Average 94 stars, based on 14 article reviews

human cd25 il2 r alpha duoset elisa kit - by Bioz Stars, 2026-10

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Article Title: Complete Remissions of Adult T-cell Leukemia with Anti-CD25 Recombinant Immunotoxin LMB-2 and Chemotherapy to Block Immunogenicity
Article Snippet: .. The soluble CD25 assay was performed either using the Human CD25/IL2 R alpha ELISA Kit from R&D systems (Minneapolis, MN), or a lab ELISA assay with comparable results. .. For the latter, Nunc Maxi sorb plates (Sigma-Aldrich, St Louis, MO) were coated with Daclizumab (humanized anti-Tac), 0.1 ug/100 uL/well, washed with TPBS (PBS containing 0.05% Tween 20 {Sigma-Aldrich}), blocked with 300 uL/well of 1% bovine serum albumin (BSA) in PBS, washed with TPBS, incubated with patient serum diluted at least 1:10 with PBS, washed with TPBS, incubated with murine MAb 7g7 in PBS, washed with TPBS, incubated with biotinylated goat anti-mouse MAb (Jackson ImmunoResearch, West Grove, PA), washed with TPBS, incubated with Avidine-HRP conjugate (Biosource, San Diego, CA) diluted 1:2500 with PBS containing 1% BSA and 0.05% Tween 20, washed with TPBS followed by PBS, incubated 15 min with TMB-peroxide substrate (Sigma-Aldrich), stopped with 2N H2SO4 and read at 450 nm.

Article Title: Complete Remissions of Adult T-cell Leukemia with Anti-CD25 Recombinant Immunotoxin LMB-2 and Chemotherapy to Block Immunogenicity
Article Snippet: .. The soluble CD25 assay was performed either using the Human CD25/IL2 R alpha ELISA Kit from R&D Systems, or a lab ELISA assay with comparable results. .. For the latter, Nunc Maxi sorb plates (Sigma-Aldrich) were coated with daclizumab (humanized anti-Tac), 0.1 μg/100 μL/well, washed with TPBS (PBS containing 0.05% Tween 20 (Sigma-Aldrich)], blocked with 300 μL/well of 1% BSA in PBS, washed with TPBS, incubated with patient serum diluted at least 1:10 with PBS, washed with TPBS, incubated with murine mAb 7g7 in PBS, washed with TPBS, incubated with biotinylated goat anti-mouse mAb (Jackson ImmunoResearch), washed with TPBS, incubated with Avidine–HRP conjugate (Biosource) diluted 1:2,500 with PBS containing 1% BSA and 0.05% Tween 20, washed with TPBS followed by PBS, incubated 15 minutes with TMB-peroxide substrate (Sigma-Aldrich), stopped with 2N H 2 SO 4 and read at 450 nm.

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human cd25 il2 r alpha duoset elisa kit (R&D Systems)

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